![]() ![]() Medical and pharmaceutical personnel are constantly dealing with dosages that require concentration measurements and dilutions. The number (concentration) of viable microbial organisms is estimated from a single dilution plate (assay) without a need for replicate plates. You will perform a serial dilution using a stock solution of 1 methylene blue dye. ![]() A calibration curve can be produced from a serial dilution, allowing the concentration of an unknown solution to be determined. How to Perform Serial Dilutions in Microbiology Microbiologics 4.58K subscribers Subscribe 3.3K 294K views 7 years ago Dr. A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony. Exercise: Practicing a Serial Dilution Before you begin this exercise, watch the video linked to the online syllabus, Serial Dilution. Of course, the addition of the stock solution affects the total volume of the diluted solution, but the final concentration is likely close enough even for medical purposes. Summary A serial dilution is prepared from a solution of known concentration, diluting by the same factor each time. When a sample diluted 1/100 is added to a sample diluted 1/10, the final dilution would be: (1/100) x (1/10) 1/1000. Serial dilutions are the culmination of a number of diluted tubes used in order to get smaller dilutions. If the stock solution is 10.0% KCl and the final volume and concentration need to be 100 mL and 0.50%, respectively, then it is easy to calculate how much stock solution to use: After you have calculated the individual dilutions for each tube, multiply the dilutions when using serial dilutions. The ASSIST is a highly capable, easy-to-use, affordable automated pipetting platform designed to perform serial dilutions in microplates. Medical personnel commonly must perform dilutions for IV solutions. Automating a serial dilution on a 96 well plate with ASSIST. ![]()
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